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KMID : 1094720000050030207
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Biotechnology and Bioprocess Engineering
2000 Volume.5 No. 3 p.207 ~ p.210
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Culture of human umbilical vein endothelial cells using 96-well microplates and position effects on cell growth
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Lee Soo-Hyun
Sohn In-Sook
Park Myung-Jin
Park In-Chul
Sohn Young-Sook
Hong Seok-Il
Choe Tae-Boo
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Abstract
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When endothelial cells isolated from human umbilical veins were cultured for 6 days using 96-well microplates, the final cell density in each well was found not to be the same although the medium composition of each well was exactly the same. Cell growth in the wells located at the periphery of a microplate was low, while that in the central area of the plate was high. A possible cause for different rate of growth was proposed as the uneven concentration of oxygen in the culture medium of each well.
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KEYWORD
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endothelial cell, culture, 96-well plate, oxygen concentration
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